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TCTP/TPT1 Rabbit pAb (A13367)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - TCTP/TPT1 Rabbit pAb (A13367)

Western blot analysis of extracts of various cell lines, using TCTP/TCTP/TPT1 antibody (A13367) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - TCTP/TPT1 Rabbit pAb (A13367)

Immunofluorescence analysis of L929 cells using TCTP/TCTP/TPT1 antibody (A13367) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameTCTP/TPT1 Rabbit pAb
Catalog No.A13367
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that is a regulator of cellular growth and proliferation. Its mRNA is highly structured and contains an oligopyrimidine tract (5'-TOP) in its 5' untranslated region that functions to repress its translation under quiescent conditions. The encoded protein is involved in a variety of cellular pathways, including apoptosis, protein synthesis and cell division. It binds to and stabilizes microtubules, and removal of this protein through phosphorylation is required for progression through mitotic and meiotic cell divisions. This gene is known to play a role in carcinogenesis, and is upregulated in some cancer cells. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human TCTP/TCTP/TPT1 (NP_003286.1).
SequenceMIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC
Gene ID
Swiss Prot
SynonymsHRF; p02; p23; TCTP
Calculated MW20kDa
Observed MW20kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesK-562, THP-1, Mouse kidney
Cellular locationCytoplasm

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ABclonal:Western blot - TCTP/TPT1 Rabbit pAb (A13367)}

Western blot - TCTP/TPT1 Rabbit pAb (A13367)

Western blot analysis of extracts of various cell lines, using TCTP/TCTP/TPT1 antibody (A13367) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - TCTP/TPT1 Rabbit pAb (A13367)}

Immunofluorescence - TCTP/TPT1 Rabbit pAb (A13367)

Immunofluorescence analysis of L929 cells using TCTP/TCTP/TPT1 antibody (A13367) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)