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TDO2 Rabbit pAb (A6766)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - TDO2 Rabbit pAb (A6766)

Western blot analysis of lysates from HUH-7 cells, using TDO2 Rabbit pAb (A6766) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - TDO2 Rabbit pAb (A6766)

Immunofluorescence analysis of A-431 cells using TDO2 Rabbit pAb (A6766) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameTDO2 Rabbit pAb
Catalog No.A6766
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human TDO2 (NP_005642.1).
SequenceGHVRDERNMLKVVSRMHRVSVILKLLVQQFSILETMTALDFNDFREYLSPASGFQSLQFRLLENKIGVLQNMRVPYNRRHYRDNFKGEENELLLKSEQEKT
Gene ID
Swiss Prot
SynonymsTO; TDO; TPH2; TRPO; HYPTRP; TDO2
Calculated MW48kDa
Observed MW48kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHUH-7
Cellular locationcytosol.
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - TDO2 Rabbit pAb (A6766)}

Western blot - TDO2 Rabbit pAb (A6766)

Western blot analysis of lysates from HUH-7 cells, using TDO2 Rabbit pAb (A6766) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - TDO2 Rabbit pAb (A6766)}

Immunofluorescence - TDO2 Rabbit pAb (A6766)

Immunofluorescence analysis of A-431 cells using TDO2 Rabbit pAb (A6766) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)