제품 > 항체 > 다중클론항체(pAb)

TEAD2 Rabbit pAb (A15594)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from A-431 cells, using TEAD2 Rabbit pAb (A15594) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	TEAD2 Rabbit pAb
Catalog No.	A15594
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables several functions, including DNA-binding transcription factor activity; disordered domain specific binding activity; and transcription coactivator binding activity. Involved in hippo signaling; positive regulation of transcription, DNA-templated; and protein-containing complex assembly. Located in cytosol and nucleoplasm. Part of TEAD-YAP complex.

Immunogen information

Immunogen	Recombinant protein of human TEAD2
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	ETF; TEF4; TEF-4; TEAD-2; TEAD2
Calculated MW	49kDa
Observed MW	51kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	A-431
Cellular location	cytosol, nucleoplasm, nucleus.
Customer validation	WB(Rattus norvegicus, Mus musculus) Co-IP(Homo sapiens) IF(Mus musculus) ChIP(Mus musculus) IP(Mus musculus)