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TERT Rabbit pAb (A16625)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Immunohistochemistry - TERT Rabbit pAb (A16625)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using TERT Rabbit pAb (A16625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameTERT Rabbit pAb
Catalog No.A16625
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1050-1132 of human TERT (NP_937983.2).
SequenceKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKLPGTTLTALEAAANPALPSDFKTILD
Gene ID
Swiss Prot
SynonymsTP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1; TERT
Calculated MW127kDa
Observed MWRefer to figures
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples
Cellular locationChromosome, Cytoplasm, Nucleus, PML body, nucleolus, nucleoplasm, telomere
Customer validation

WB(Homo sapiens, Mus musculus )

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ABclonal:Immunohistochemistry - TERT Rabbit pAb (A16625)}

Immunohistochemistry - TERT Rabbit pAb (A16625)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using TERT Rabbit pAb (A16625) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A16625? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

ELISA 키트 (1)

Secondary Antibodies (26)