Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using TGF beta Receptor I (TGFBR1) Rabbit pAb (A16983) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
| Product name | TGF beta Receptor I (TGFBR1) Rabbit pAb |
|---|---|
| Catalog No. | A16983 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 161-179 of human TGF beta Receptor I (TGFBR1) (NP_004603.1). |
|---|---|
| Sequence | EEDPSLDRPFISEGTTLKD |
| Gene ID | |
| Swiss Prot | |
| Synonyms | AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; TBRI; ALK-5; LDS1A; LDS2A; TBR-i; TGFR-1; ACVRLK4; tbetaR-I; TGF beta Receptor I (TGFBR1) |
| Calculated MW | 56kDa |
| Observed MW | 56kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | PC-3, Mouse lung |
| Cellular location | Cell junction, Cell membrane, Single-pass type I membrane protein, tight junction. |
| Customer validation | WB(Rattus norvegicus, Homo sapiens, Mus musculus, Mus musculus) IF(Homo sapiens, Mus musculus) IF(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A16983? Please let us know so that we can cite the reference in this datasheet.
