제품 > 항체 > 다중클론항체(pAb)

THADA Rabbit pAb (A7129)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - THADA Rabbit pAb (A7129)

Western blot analysis of extracts of various cell lines, using THADA antibody (A7129) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - THADA Rabbit pAb (A7129)

Immunofluorescence analysis of A549 cells using THADA antibody (A7129). Blue: DAPI for nuclear staining.

Overview

Product nameTHADA Rabbit pAb
Catalog No.A7129
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is the target of 2p21 choromosomal aberrations in benign thyroid adenomas. Single nucleotide polymorphisms (SNPs) in this gene may be associated with type 2 diabetes and polycystic ovary syndrome. The encoded protein is likely involved in the death receptor pathway and apoptosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1684-1953 of human THADA (NP_071348.3).
SequenceQLVILSCEDHLPTESRLAVVEVLTSTTPLFLTNPHPILELQDTLALWKCVLTLLQSEEQAVRDAATETVTTAMSQENTCQSTEFAFCQVDASIALALALAVLCDLLQQWDQLAPGLPILLGWLLGESDDLVACVESMHQVEEDYLFEKAEVNFWAETLIFVKYLCKHLFCLLSKSGWRPPSPEMLCHLQRMVSEQCHLLSQFFRELPPAAEFVKTVEFTRLRIQEERTLACLRLLAFLEGKEGEDTLVLSVWDSYAESRQLTLPRTEAAC
Gene ID
Swiss Prot
SynonymsGITA; ARMC13; Trm732
Calculated MW220kDa
Observed MW220kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7, HL-60, U-87MG, NCI-H460, Mouse lung, Mouse testis
Cellular location

Documents

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    ABclonal:Western blot - THADA Rabbit pAb (A7129)}

    Western blot - THADA Rabbit pAb (A7129)

    Western blot analysis of extracts of various cell lines, using THADA antibody (A7129) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - THADA Rabbit pAb (A7129)}

    Immunofluorescence - THADA Rabbit pAb (A7129)

    Immunofluorescence analysis of A549 cells using THADA antibody (A7129). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)