제품 > 항체 > 다중클론항체(pAb)

THBS3 Rabbit pAb (A3641)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - THBS3 Rabbit pAb (A3641)

Western blot analysis of extracts of various cell lines, using THBS3 antibody (A3641) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameTHBS3 Rabbit pAb
Catalog No.A3641
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 787-956 of human THBS3 (NP_009043.1).
SequenceTDDDYAGFLFSYQDSGRFYVVMWKQTEQTYWQATPFRAVAQPGLQLKAVTSVSGPGEHLRNALWHTGHTPDQVRLLWTDPRNVGWRDKTSYRWQLLHRPQVGYIRVKLYEGPQLVADSGVIIDTSMRGGRLGVFCFSQENIIWSNLQYRCNDTVPEDFEPFRRQLLQGRV
Gene ID
Swiss Prot
SynonymsTSP3
Calculated MW104kDa
Observed MW104kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse kidney, Mouse ovary
Cellular locationextracellular region, perinuclear region of cytoplasm
ABclonal:Western blot - THBS3 Rabbit pAb (A3641)}

Western blot - THBS3 Rabbit pAb (A3641)

Western blot analysis of extracts of various cell lines, using THBS3 antibody (A3641) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)