제품 > 항체 > 다중클론항체(pAb)

TIMD4 Rabbit pAb (A17807)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - TIMD4 Rabbit pAb (A17807)

Western blot analysis of lysates from U-87MG cells, using TIMD4 Rabbit pAb (A17807) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - TIMD4 Rabbit pAb (A17807)

Western blot analysis of lysates from Rat spleen, using TIMD4 Rabbit pAb (A17807) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 300s.

Overview

Product nameTIMD4 Rabbit pAb
Catalog No.A17807
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable phosphatidylserine binding activity. Predicted to act upstream of or within apoptotic cell clearance. Predicted to be integral component of membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-130 of human TIMD4 (NP_612388.2).
SequenceETVVTEVLGHRVTLPCLYSSWSHNSNSMCWGKDQCPYSGCKEALIRTDGMRVTSRKSAKYRLQGTIPRGDVSLTILNPSESDSGVYCCRIEVPGWFNDVKINVRLN
Gene ID
Swiss Prot
SynonymsTIM4; SMUCKLER; TIMD4
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, Rat spleen
Cellular locationextracellular region, plasma membrane
    ABclonal:Western blot - TIMD4 Rabbit pAb (A17807)}

    Western blot - TIMD4 Rabbit pAb (A17807)

    Western blot analysis of lysates from U-87MG cells, using TIMD4 Rabbit pAb (A17807) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - TIMD4 Rabbit pAb (A17807)}

    Western blot - TIMD4 Rabbit pAb (A17807)

    Western blot analysis of lysates from Rat spleen, using TIMD4 Rabbit pAb (A17807) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 300s.

    * For research use only. Not for therapeutic or diagnostic purposes.