제품 > 항체 > 단일클론항체(mAb)

TIMM22 Rabbit mAb (A23557)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TIMM22 Rabbit mAb (A23557)

Western blot analysis of lysates from HepG2 cells, using TIMM22 Rabbit mAb (A23557) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - TIMM22 Rabbit mAb (A23557)

Western blot analysis of various lysates, using TIMM22 Rabbit mAb (A23557) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameTIMM22 Rabbit mAb
Catalog No.A23557
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3118
Multipass transmembrane proteins are brought into mitochondria and inserted into the mitochondrial inner membrane by way of the TIM22 complex. This complex has six subunits and is a twin-pore translocase. The protein encoded by this gene is a subunit of TIM22 and represents the voltage-activated and signal-gated channel.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 20-120aa of human TIMM22.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsTEX4; TIM22; COXPD43; TIMM22
Calculated MW20kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHep G2, Mouse ovary, Rat heart
Cellular location

Documents

Certificate of Compliance

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ABclonal:Western blot - TIMM22 Rabbit mAb (A23557)}

Western blot - TIMM22 Rabbit mAb (A23557)

Western blot analysis of lysates from HepG2 cells, using TIMM22 Rabbit mAb (A23557) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - TIMM22 Rabbit mAb (A23557)}

Western blot - TIMM22 Rabbit mAb (A23557)

Western blot analysis of various lysates, using TIMM22 Rabbit mAb (A23557) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.