제품 > 항체 > 다중클론항체(pAb)

TIR1 Rabbit pAb (A21276)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Arabidopsis thaliana

ABclonal:Western blot - TIR1 Rabbit pAb (A21276)

Western blot analysis of lysates from Inflorescences, using TIR1 Rabbit pAb (A21276) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameTIR1 Rabbit pAb
Catalog No.A21276
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Encodes an auxin receptor that mediates auxin-regulated transcription. It contains leucine-rich repeats and an F-box and interacts with ASK1, ASK2 and AtCUL1 to form SCF-TIR1, an SCF ubiquitin ligase complex. Related to yeast Grr1p and human SKP2 proteins, involved in ubiquitin-mediated processes. Required for normal response to auxin and repressed in response to flagellin. As part of the SCF complex and in the presence of auxin, TIR1 interacts with Aux/IAA transcriptional repressor proteins and mediates their degradation. Mutations in TIR1 block auxin stimulation of flavonoid synthesis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 495-594 of arabidopsis thaliana TIR1 (NP_567135.1).
SequenceDKALLANASKLETMRSLWMSSCSVSFGACKLLGQKMPKLNVEVIDERGAPDSRPESCPVERVFIYRTVAGPRFDMPGFVWNMDQDSTMRFSRQIITTNGL
Gene ID
Swiss Prot
SynonymsAtTIR1; TRANSPORT INHIBITOR RESPONSE 1; TIR1
Calculated MW67kDa
Observed MW67kDa
ReactivityArabidopsis thaliana
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesInflorescences
Cellular locationnucleus

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    ABclonal:Western blot - TIR1 Rabbit pAb (A21276)}

    Western blot - TIR1 Rabbit pAb (A21276)

    Western blot analysis of lysates from Inflorescences, using TIR1 Rabbit pAb (A21276) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.