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TLE2 Rabbit mAb (A23547)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TLE2 Rabbit mAb (A23547)

Western blot analysis of various lysates, using TLE2 Rabbit mAb (A23547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - TLE2 Rabbit mAb (A23547)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using TLE2 Rabbit mAb (A23547) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TLE2 Rabbit mAb (A23547)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using TLE2 Rabbit mAb (A23547) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameTLE2 Rabbit mAb
Catalog No.A23547
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61201
Enables transcription corepressor activity. Involved in negative regulation of canonical Wnt signaling pathway and negative regulation of transcription, DNA-templated. Located in focal adhesion and nuclear body.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human TLE2. (NP_003251.2).
SequenceMYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQLQPLSHHAPPVPL
Gene ID
Swiss Prot
SynonymsESG; ESG2; GRG2; TLE2
Calculated MW80KDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesK-562, MCF7, SH-SY5Y
Cellular locationNucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - TLE2 Rabbit mAb (A23547)}

Western blot - TLE2 Rabbit mAb (A23547)

Western blot analysis of various lysates, using TLE2 Rabbit mAb (A23547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - TLE2 Rabbit mAb (A23547)}

Immunohistochemistry - TLE2 Rabbit mAb (A23547)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using TLE2 Rabbit mAb (A23547) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TLE2 Rabbit mAb (A23547)}

Immunohistochemistry - TLE2 Rabbit mAb (A23547)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using TLE2 Rabbit mAb (A23547) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)