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TLK1 Rabbit pAb (A14831)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TLK1 Rabbit pAb (A14831)

Western blot analysis of various lysates using TLK1 Rabbit pAb (A14831) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - TLK1 Rabbit pAb (A14831)

Immunohistochemistry analysis of paraffin-embedded Rat heart using TLK1 Rabbit pAb (A14831) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - TLK1 Rabbit pAb (A14831)

Immunohistochemistry analysis of paraffin-embedded Human placenta using TLK1 Rabbit pAb (A14831) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - TLK1 Rabbit pAb (A14831)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using TLK1 Rabbit pAb (A14831) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameTLK1 Rabbit pAb
Catalog No.A14831
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 160-230 of human TLK1 (NP_036422.3).
SequencePVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQ
Gene ID
Swiss Prot
SynonymsPKU-beta; TLK1
Calculated MW87kDa
Observed MW87kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat, NIH/3T3
Cellular locationNucleus.

Documents

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    ABclonal:Western blot - TLK1 Rabbit pAb (A14831)}

    Western blot - TLK1 Rabbit pAb (A14831)

    Western blot analysis of various lysates using TLK1 Rabbit pAb (A14831) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - TLK1 Rabbit pAb (A14831)}

    Immunohistochemistry - TLK1 Rabbit pAb (A14831)

    Immunohistochemistry analysis of paraffin-embedded Rat heart using TLK1 Rabbit pAb (A14831) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - TLK1 Rabbit pAb (A14831)}

    Immunohistochemistry - TLK1 Rabbit pAb (A14831)

    Immunohistochemistry analysis of paraffin-embedded Human placenta using TLK1 Rabbit pAb (A14831) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - TLK1 Rabbit pAb (A14831)}

    Immunohistochemistry - TLK1 Rabbit pAb (A14831)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using TLK1 Rabbit pAb (A14831) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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