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TLR2 Rabbit pAb (A11225)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Rat lung, using TLR2 Rabbit pAb (A11225) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - TLR2 Rabbit pAb (A11225)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using TLR2 Rabbit pAb (A11225) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse islets of langerhans using TLR2 Rabbit pAb (A11225) at dilution of 1:150 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - TLR2 Rabbit pAb (A11225)

Immunofluorescence analysis of THP-1 cells using TLR2 Rabbit pAb (A11225) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	TLR2 Rabbit pAb
Catalog No.	A11225
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This protein is a cell-surface protein that can form heterodimers with other TLR family members to recognize conserved molecules derived from microorganisms known as pathogen-associated molecular patterns (PAMPs). Activation of TLRs by PAMPs leads to an up-regulation of signaling pathways to modulate the host's inflammatory response. This gene is also thought to promote apoptosis in response to bacterial lipoproteins. This gene has been implicated in the pathogenesis of several autoimmune diseases. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 600-700 of human TLR2 (NP_003255.2).
Sequence	LLILLTGVLCHRFHGLWYMKMMWAWLQAKRKPRKAPSRNICYDAFVSYSERDAYWVENLMVQELENFNPPFKLCLHKRDFIPGKWIIDNIIDSIEKSHKTV
Gene ID
Swiss Prot
Synonyms	TIL4; CD282; TLR2
Calculated MW	90kDa
Observed MW	105kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Rat lung
Cellular location	Membrane, Single-pass type I membrane protein.
Customer validation	IF(Rattus norvegicus, Mus musculus) WB(Mus musculus, Sus scrofa, Homo sapiens, Mus musculus, Rattus norvegicus) IHC(Homo sapiens, Rattus norvegicus) IF(Mus musculus) IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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Research Area

TLR2 Rabbit pAb images

Western blot - TLR2 Rabbit pAb (A11225)

Immunohistochemistry - TLR2 Rabbit pAb (A11225)

Immunofluorescence - TLR2 Rabbit pAb (A11225)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Frontiers in Immunology

Application:

IF:

5.7

Species:

Rattus norvegicus

PMID:

31956324

Title:

Therapeutic Effect of Bifidobacterium Administration on Experimental Autoimmune Myasthenia Gravis in Lewis Rats.

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Theranostics

Application:

IF:

12.4

Species:

Mus musculus

PMID:

33500729

Title:

An electroporation strategy to synthesize the membrane-coated nanoparticles for enhanced anti-inflammation therapy in bone infection

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Int J Ophthalmol

Application:

IF:

1.9

Species:

Mus musculus

PMID:

33214996

Title:

Anti-inflammatory effects of astaxanthin against fungal keratitis

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Int Immunopharmacol

Application:

IF:

4.8

Species:

Mus musculus

PMID:

33550031

Title:

Quercetin amelioratesAspergillus fumigatuskeratitis by inhibiting fungal growth, toll-like receptors and inflammatory cytokines

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Brain

Application:

IF:

10.6

Species:

Mus musculus

PMID:

33792662

Title:

Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Vet Immunol Immunopathol

Application:

IF:

1.4

Species:

Sus scrofa

PMID:

34461425

Title:

Clostridium butyricum CB1 up-regulates FcRn expression via activation of TLR2/4-NF-κB signaling pathway in porcine small intestinal cells

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Toxicol Appl Pharmacol

Application:

IF:

3.3

Species:

Mus musculus

PMID:

34755645

Title:

Indoprofen exerts a potent therapeutic effect against sepsis by alleviating high mobility group box 1-mediated inflammatory responses

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

3.9

Species:

Mus musculus

PMID:

37269894

Title:

Intestinal microbiota regulates colonic inflammation in fluorosis mice by TLR/NF-κB pathway through short-chain fatty acids

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Investigative Ophthalmology & Visual Science

Application:

IF:

Species:

Homo sapiens, Mus musculus

PMID:

32347916

Title:

Honokiol Reduces Fungal Load, Toll-Like Receptor-2, and Inflammatory Cytokines in Aspergillus fumigatus Keratitis

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

WB, IHC

IF:

5.6

Species:

Homo sapiens

PMID:

36293206

Title:

Immunomodulatory Effect of Human Lactoferrin on Toll-like Receptors 2 Expression as Therapeutic Approach for Keratoconus - PMC

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Journal of Molecular and Cellular Cardiology

Application:

WB, IF

IF:

4.9

Species:

Mus musculus

PMID:

32278833

Title:

MiR-144-5p limits experimental abdominal aortic aneurysm formation by mitigating M1 macrophage-associated inflammation: Suppression of TLR2 and OLR1

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Journal of neuroinflammation

Application:

WB, IHC

IF:

9.6

Species:

Rattus norvegicus

PMID:

36788559

Title:

-Synuclein induces prodromal symptoms of Parkinsons disease via activating TLR2/MyD88/NF-B pathway in Schwann cells of vagus nerve in a rat model - PMC

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Frontiers in bioscience (Scholar edition)

Application:

WB, IF

IF:

Species:

Mus musculus

PMID:

36137979

Title:

Inducible Heat Shock Protein 70 Levels in Patients and the mdx Mouse Affirm Regulation during Skeletal Muscle Regeneration in Muscular Dystrophy

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Cells

Application:

WB, IHC

IF:

7.7

Species:

Rattus norvegicus

PMID:

36359764

Title:

Hyaluronan Regulates Neuronal and Immune Function in the Rat Small Intestine and Colonic Microbiota after Ischemic/Reperfusion Injury - PMC

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

ACS nano

Application:

IF:

15.8

Species:

Mus musculus

PMID:

36715347

Title:

Dual-Targeted Nanodiscs Revealing the Cross-Talk between Osteogenic Differentiation of Mesenchymal Stem Cells and Macrophages

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Frontiers in Physiology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

31680990

Title:

Dysbiosis-Associated Enteric Glial Cell Immune-Activation and Redox Imbalance Modulate Tight Junction Protein Expression in Gulf War Illness Pathology.

References for TLR2 Rabbit pAb (A11225)

Product:

TLR2 Rabbit pAb

Journal:

Environmental pollution (Barking, Essex : 1987)

Application:

IHC

IF:

7.6

Species:

Rattus norvegicus

PMID: