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TLR5 Rabbit pAb (A1721)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - TLR5 Rabbit pAb (A1721)

Western blot analysis of lysates from HeLa cells, using TLR5 Rabbit pAb (A1721).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - TLR5 Rabbit pAb (A1721)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TLR5 Rabbit pAb (A1721) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TLR5 Rabbit pAb (A1721)

Immunohistochemistry analysis of paraffin-embedded human tonsil using TLR5 Rabbit pAb (A1721) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameTLR5 Rabbit pAb
Catalog No.A1721
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 714-858 of human TLR5 (NP_003259.2).
SequenceHLDTQYSDQNRFNLCFEERDFVPGENRIANIQDAIWNSRKIVCLVSRHFLRDGWCLEAFSYAQGRCLSDLNSALIMVVVGSLSQYQLMKHQSIRGFVQKQQYLRWPEDLQDVGWFLHKLSQQILKKEKEKKKDNNIPLQTVATIS
Gene ID
Swiss Prot
SynonymsSLE1; TIL3; SLEB1; MELIOS; TLR5
Calculated MW98kDa
Observed MW100kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular locationMembrane, Single-pass type I membrane protein

Documents

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ABclonal:Western blot - TLR5 Rabbit pAb (A1721)}

Western blot - TLR5 Rabbit pAb (A1721)

Western blot analysis of lysates from HeLa cells, using TLR5 Rabbit pAb (A1721).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - TLR5 Rabbit pAb (A1721)}

Immunohistochemistry - TLR5 Rabbit pAb (A1721)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TLR5 Rabbit pAb (A1721) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TLR5 Rabbit pAb (A1721)}

Immunohistochemistry - TLR5 Rabbit pAb (A1721)

Immunohistochemistry analysis of paraffin-embedded human tonsil using TLR5 Rabbit pAb (A1721) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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