제품 > 항체 > 다중클론항체(pAb)

TLR8 Rabbit pAb (A1489)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TLR8 Rabbit pAb (A1489)

Western blot analysis of lysates from HepG2 cells, using TLR8 Rabbit pAb (A1489) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TLR8 Rabbit pAb (A1489)

Immunohistochemistry analysis of paraffin-embedded Human colon using TLR8 Rabbit pAb (A1489) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TLR8 Rabbit pAb (A1489)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using TLR8 Rabbit pAb (A1489) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TLR8 Rabbit pAb (A1489)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TLR8 Rabbit pAb (A1489) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - TLR8 Rabbit pAb (A1489)

Immunofluorescence analysis of paraffin-embedded rat spleen using TLR8 Rabbit pAb (A1489) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TLR8 Rabbit pAb (A1489)

Immunofluorescence analysis of paraffin-embedded mouse spleen using TLR8 Rabbit pAb (A1489) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameTLR8 Rabbit pAb
Catalog No.A1489
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 849-1041 of human TLR8 (NP_619542.1).
SequenceHHLFYWDVWFIYNVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRYHLEESRDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTVFVLTKKYAKSWNFKTAFYLALQRLMDENMDVIIFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQTLRNVVLTENDSRYNNMYVDSIKQY
Gene ID
Swiss Prot
SynonymsCD288; IMD98; hTLR8; TLR8
Calculated MW120kDa
Observed MW150kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2
Cellular locationMembrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - TLR8 Rabbit pAb (A1489)}

Western blot - TLR8 Rabbit pAb (A1489)

Western blot analysis of lysates from HepG2 cells, using TLR8 Rabbit pAb (A1489) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - TLR8 Rabbit pAb (A1489)}

Immunohistochemistry - TLR8 Rabbit pAb (A1489)

Immunohistochemistry analysis of paraffin-embedded Human colon using TLR8 Rabbit pAb (A1489) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TLR8 Rabbit pAb (A1489)}

Immunohistochemistry - TLR8 Rabbit pAb (A1489)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using TLR8 Rabbit pAb (A1489) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TLR8 Rabbit pAb (A1489)}

Immunohistochemistry - TLR8 Rabbit pAb (A1489)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TLR8 Rabbit pAb (A1489) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - TLR8 Rabbit pAb (A1489)}

Immunofluorescence - TLR8 Rabbit pAb (A1489)

Immunofluorescence analysis of paraffin-embedded rat spleen using TLR8 Rabbit pAb (A1489) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TLR8 Rabbit pAb (A1489)}

Immunofluorescence - TLR8 Rabbit pAb (A1489)

Immunofluorescence analysis of paraffin-embedded mouse spleen using TLR8 Rabbit pAb (A1489) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)