제품 > 항체 > 다중클론항체(pAb)

TM9SF1 Rabbit pAb (A7461)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - TM9SF1 Rabbit pAb (A7461)

Western blot analysis of extracts of various cell lines, using TM9SF1 antibody (A7461) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameTM9SF1 Rabbit pAb
Catalog No.A7461
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in protein localization to membrane. Predicted to be located in autophagosome membrane; cytoplasmic vesicle; and lysosomal membrane. Predicted to be integral component of membrane. Predicted to be active in membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 28-230 of human TM9SF1 (NP_006396.2).
SequencePGVEGVTHYKAGDPVILYVNKVGPYHNPQETYHYYQLPVCCPEKIRHKSLSLGEVLDGDRMAESLYEIRFRENVEKRILCHMQLSSAQVEQLRQAIEELYYFEFVVDDLPIRGFVGYMEESGFLPHSHKIGLWTHLDFHLEFHGDRIIFANVSVRDVKPHSLDGLRPDEFLGLTHTYSVRWSETSVERRSDRRRGDDGGFFPR
Gene ID
Swiss Prot
SynonymsMP70; HMP70
Calculated MW69kDa
Observed MW80kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNCI-H460, U-251MG, SW620, Mouse liver
Cellular locationCytoplasmic vesicle, Lysosome membrane, Multi-pass membrane protein, autophagosome membrane

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    ABclonal:Western blot - TM9SF1 Rabbit pAb (A7461)}

    Western blot - TM9SF1 Rabbit pAb (A7461)

    Western blot analysis of extracts of various cell lines, using TM9SF1 antibody (A7461) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)