제품 > 항체 > 다중클론항체(pAb)

TMED2 Rabbit pAb (A17622)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using TMED2 Rabbit pAb (A17622) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunofluorescence - TMED2 Rabbit pAb (A17622)

Immunofluorescence analysis of C6 cells using TMED2 Rabbit pAb (A17622) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using TMED2 Rabbit pAb (A17622) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	TMED2 Rabbit pAb
Catalog No.	A17622
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Involved in several processes, including Golgi organization; negative regulation of GTPase activity; and protein localization to plasma membrane. Located in Golgi apparatus; endoplasmic reticulum; and endoplasmic reticulum-Golgi intermediate compartment.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 60-170 of human TMED2 (NP_006806.1).
Sequence	EITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVV
Gene ID
Swiss Prot
Synonyms	p24; P24A; RNP24; p24b1; p24beta1; TMED2
Calculated MW	23kDa
Observed MW	23kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	U-87MG, HepG2, Mouse liver, Mouse kidney, Rat spleen, Rat lung, Rat liver
Cellular location	COPI-coated vesicle, COPII-coated ER to Golgi transport vesicle, endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, transport vesicle, zymogen granule membrane
Customer validation	WB(Chlorocebus aethiops )