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TMED9 Rabbit pAb (A3442)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TMED9 Rabbit pAb (A3442)

Western blot analysis of various lysates using TMED9 Rabbit pAb (A3442) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameTMED9 Rabbit pAb
Catalog No.A3442
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of a family of genes encoding transport proteins located in the endoplasmic reticulum and the Golgi. A similar gene in mouse is the target of microRNA miR-296, which is part of an imprinted cluster.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human TMED9 (NP_059980.2).
SequenceMAVELGVLLVRPRPGTGLGRVMRTLLLVLWLATRGSALYFHIGETEKKCFIEEIPDETMVIGNYRTQLYDKQREEYQPATPGLGMFVEVKDPEDKVILARQYGSEGRFTFTSHTPGEHQICLHSNSTKFSLFAGGMLRVHLDIQVGEHANDYAEIAAKDKLSELQLRVRQLVEQVEQIQKEQNYQRWREERFRQTSESTNQRVLWWSILQTLILVAIGVWQMRH
Gene ID
Swiss Prot
Synonymsp25; GMP25; p24a2; HSGP25L2G; p24alpha2; TMED9
Calculated MW27kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-431, HeLa, Raji, Mouse liver, Mouse brain, Mouse lung, Rat liver
Cellular locationEndoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus, Single-pass type I membrane protein, cis-Golgi network membrane, trans-Golgi network membrane
Customer validation

WB(Homo sapiens)

Documents

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    ABclonal:Western blot - TMED9 Rabbit pAb (A3442)}

    Western blot - TMED9 Rabbit pAb (A3442)

    Western blot analysis of various lysates using TMED9 Rabbit pAb (A3442) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A3442? Please let us know so that we can cite the reference in this datasheet.