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TMEM119 Rabbit mAb (A27143)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - TMEM119 Rabbit mAb (A27143)

Western blot analysis of lysates from Mouse brain using TMEM119 Rabbit mAb (A27143) at 1:12000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - TMEM119 Rabbit mAb (A27143)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using TMEM119 Rabbit mAb (A27143) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TMEM119 Rabbit mAb (A27143)

Immunohistochemistry analysis of paraffin-embedded Mouse intestine tissue using TMEM119 Rabbit mAb (A27143) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - TMEM119 Rabbit mAb (A27143)

Confocal imaging of paraffin-embedded Mouse brain tissue using TMEM119 Rabbit mAb (A27143, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameTMEM119 Rabbit mAb
Catalog No.A27143
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5161
Involved in positive regulation of bone mineralization; positive regulation of osteoblast differentiation; and positive regulation of osteoblast proliferation. Located in plasma membrane.
ImmunogenRecombinant protein of human TMEM119.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsOBIF
Calculated MW29kDa
Observed MW56kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:6000 - 1:24000
  • IHC-P 1:2000 - 1:8000
  • IF/ICC 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain
Cellular locationplasma membrane.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - TMEM119 Rabbit mAb (A27143)}

    Western blot - TMEM119 Rabbit mAb (A27143)

    Western blot analysis of lysates from Mouse brain using TMEM119 Rabbit mAb (A27143) at 1:12000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.
    ABclonal:Immunohistochemistry - TMEM119 Rabbit mAb (A27143)}

    Immunohistochemistry - TMEM119 Rabbit mAb (A27143)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using TMEM119 Rabbit mAb (A27143) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - TMEM119 Rabbit mAb (A27143)}

    Immunohistochemistry - TMEM119 Rabbit mAb (A27143)

    Immunohistochemistry analysis of paraffin-embedded Mouse intestine tissue using TMEM119 Rabbit mAb (A27143) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - TMEM119 Rabbit mAb (A27143)}

    Immunofluorescence - TMEM119 Rabbit mAb (A27143)

    Confocal imaging of paraffin-embedded Mouse brain tissue using TMEM119 Rabbit mAb (A27143, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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