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TMEM119 Rabbit pAb (A22118)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - TMEM119 Rabbit pAb (A22118)

Western blot analysis of various lysates, using TMEM119 Rabbit pAb (A22118) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

Overview

Product nameTMEM119 Rabbit pAb
Catalog No.A22118
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in positive regulation of bone mineralization; positive regulation of osteoblast differentiation; and positive regulation of osteoblast proliferation. Located in plasma membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 160-270 of human TMEM119 (NP_859075.2).
SequenceSRPEEALDSSRQLQADILAATQNLKSPTRAALGGGDGARMVEGRGAEEEEKGSQEGDQEVQGHGVPVETPEAQEEPCSGVLEGAVVAGEGQGELEGSLLLAQEAQGPVGPP
Gene ID
Swiss Prot
SynonymsOBIF
Calculated MW29kDa
Observed MW29kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse lung, Rat lung
Cellular locationplasma membrane
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - TMEM119 Rabbit pAb (A22118)}

    Western blot - TMEM119 Rabbit pAb (A22118)

    Western blot analysis of various lysates, using TMEM119 Rabbit pAb (A22118) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 120s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A22118? Please let us know so that we can cite the reference in this datasheet.

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    Secondary Antibodies (26)