제품 > 항체 > 다중클론항체(pAb)

TMPRSS6 Rabbit pAb (A10642)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TMPRSS6 Rabbit pAb (A10642)

Western blot analysis of extracts of various cell lines, using TMPRSS6 antibody (A10642) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - TMPRSS6 Rabbit pAb (A10642)

Western blot analysis of extracts of Rat liver, using TMPRSS6 antibody (A10642) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - TMPRSS6 Rabbit pAb (A10642)

Immunofluorescence analysis of mouse liver cells using TMPRSS6 Rabbit pAb (A10642) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameTMPRSS6 Rabbit pAb
Catalog No.A10642
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a type II transmembrane serine proteinase that is found attached to the cell surface. The encoded protein may be involved in matrix remodeling processes in the liver. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 77-220 of human TMPRSS6 (NP_705837.1).
SequenceGYKAEVMVSQVYSGSLRVLNRHFSQDLTRRESSAFRSETAKAQKMLKELITSTRLGTYYNSSSVYSFGEGPLTCFFWFILQIPEHRRLMLSPEVVQALLVEELLSTVNSSAAVPYRAEYEVDPEGLVILEASVKDIAALNSTLG
Gene ID
Swiss Prot
SynonymsMT2; IRIDA
Calculated MW90kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesLO2, Mouse liver, Rat liver
Cellular locationCell membrane, Single-pass type II membrane protein

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - TMPRSS6 Rabbit pAb (A10642)}

    Western blot - TMPRSS6 Rabbit pAb (A10642)

    Western blot analysis of extracts of various cell lines, using TMPRSS6 antibody (A10642) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - TMPRSS6 Rabbit pAb (A10642)}

    Western blot - TMPRSS6 Rabbit pAb (A10642)

    Western blot analysis of extracts of Rat liver, using TMPRSS6 antibody (A10642) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunofluorescence - TMPRSS6 Rabbit pAb (A10642)}

    Immunofluorescence - TMPRSS6 Rabbit pAb (A10642)

    Immunofluorescence analysis of mouse liver cells using TMPRSS6 Rabbit pAb (A10642) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.