제품 > 항체 > 다중클론항체(pAb)

TNFα Rabbit pAb (A23264)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - TNFα Rabbit pAb (A23264)

Western blot analysis of Recombinant Rat TNF-alpha Protein (RP01322), using TNFα Rabbit pAb (A23264) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 5ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - TNFα Rabbit pAb (A23264)

Western blot analysis of lysates from THP-1 cells, using TNFα Rabbit pAb (A23264) at 1:2000 dilution. THP-1 cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes and LPS (1 μg/ml) at 37℃ for 6 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Western blot - TNFα Rabbit pAb (A23264)

Western blot analysis of lysates from RAW 264.7 cells, using TNFα Rabbit pAb (A23264) at 1:2000 dilution. Raw 264. 7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameTNFα Rabbit pAb
Catalog No.A23264
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 80-235 of rat TNFα (NP_036807.1).
SequenceLRSSSQNSSDKPVAHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLLSAEVNLPKYLDITESGQVYFGVIAL
Gene ID
Swiss Prot
SynonymsTnfa; RATTNF; TNF-alpha; TNFα
Calculated MW26kDa
Observed MW17kDa/26kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesTHP-1 treated by TPA and LPS, Recombinant Rat TNF-alpha Protein, Raw 264.7 treated by LPS
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - TNFα Rabbit pAb (A23264)}

    Western blot - TNFα Rabbit pAb (A23264)

    Western blot analysis of Recombinant Rat TNF-alpha Protein (RP01322), using TNFα Rabbit pAb (A23264) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 5ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - TNFα Rabbit pAb (A23264)}

    Western blot - TNFα Rabbit pAb (A23264)

    Western blot analysis of lysates from THP-1 cells, using TNFα Rabbit pAb (A23264) at 1:2000 dilution. THP-1 cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes and LPS (1 μg/ml) at 37℃ for 6 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.
    ABclonal:Western blot - TNFα Rabbit pAb (A23264)}

    Western blot - TNFα Rabbit pAb (A23264)

    Western blot analysis of lysates from RAW 264.7 cells, using TNFα Rabbit pAb (A23264) at 1:2000 dilution. Raw 264. 7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    ELISA 키트 (1)

    제품 (2)

    재조합 단백질 (2)

    Secondary Antibodies (26)