제품 > 항체 > 단일클론항체(mAb)

TOR4A Rabbit mAb (A20952)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - TOR4A Rabbit mAb (A20952)

Western blot analysis of lysates from Mouse Thymus, using TOR4A Rabbit mAb (A20952) at 1 : 1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - TOR4A Rabbit mAb (A20952)

Western blot analysis of various lysates, using TOR4A Rabbit mAb (A20952) at 1 : 1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product nameTOR4A Rabbit mAb
Catalog No.A20952
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2939
Predicted to enable ATP binding activity. Predicted to be located in extracellular region and platelet alpha granule lumen. Predicted to be active in endoplasmic reticulum lumen and nuclear envelope.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TOR4A (Q9NXH8).
SequenceMDRGQPSLEPAAAAPRASGRCVIAPVRAVLRLRRRVCVLRKRRLLQPGGGPDVGTGAPRPGCSPRAPRADLDQPKFFTFDSPAELPSRTPRKKRRRSRLVLYPETSRKYRPRVEHRSRAQRCLLLLVAIV
Gene ID
Swiss Prot
SynonymsC9orf167; TOR4A
Calculated MW47kDa
Observed MW47kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesK-562, A-549, Jurkat, mouse thymus
Cellular locationendoplasmic reticulum lumen, extracellular region, nuclear envelope

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - TOR4A Rabbit mAb (A20952)}

    Western blot - TOR4A Rabbit mAb (A20952)

    Western blot analysis of lysates from Mouse Thymus, using TOR4A Rabbit mAb (A20952) at 1 : 1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - TOR4A Rabbit mAb (A20952)}

    Western blot - TOR4A Rabbit mAb (A20952)

    Western blot analysis of various lysates, using TOR4A Rabbit mAb (A20952) at 1 : 1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.

    * For research use only. Not for therapeutic or diagnostic purposes.