제품 > 항체 > 다중클론항체(pAb)

TPP2 Rabbit pAb (A6421)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TPP2 Rabbit pAb (A6421)

Western blot analysis of extracts of various cell lines, using TPP2 antibody (A6421) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - TPP2 Rabbit pAb (A6421)

Immunofluorescence analysis of U2OS cells using TPP2 antibody (A6421). Blue: DAPI for nuclear staining.

Overview

Product nameTPP2 Rabbit pAb
Catalog No.A6421
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TPP2 (NP_003282.2).
SequenceMATAATEEPFPFHGLLPKKETGAASFLCRYPEYDGRGVLIAVLDTGVDPGAPGMQVTTDGKPKIVDIIDTTGSGDVNTATEVEPKDGEIVGLSGRVLKIPASWTNPSGKYHIGIKNGYDFYPKALKERIQKERKEKIWDPVHRVALAEACRKQEEFDVANNGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGEVWRACIDSNEDGDLSKSTVLRNYKEAQEYGSFGTAEMLNYSVNIYDDGNLLSIVTSGGAHGTHVASIAAGHFPEEPERNGVAPGAQILSIKIGDTR
Gene ID
Swiss Prot
SynonymsIMD78; TPP-2; TPPII; TPP-II
Calculated MW138kDa
Observed MW138kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat, SW620, HeLa, HepG2, Mouse brain, Mouse spleen
Cellular locationCytoplasm, Nucleus

Documents

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    ABclonal:Western blot - TPP2 Rabbit pAb (A6421)}

    Western blot - TPP2 Rabbit pAb (A6421)

    Western blot analysis of extracts of various cell lines, using TPP2 antibody (A6421) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - TPP2 Rabbit pAb (A6421)}

    Immunofluorescence - TPP2 Rabbit pAb (A6421)

    Immunofluorescence analysis of U2OS cells using TPP2 antibody (A6421). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)