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TPRG1L Rabbit pAb (A15949)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - TPRG1L Rabbit pAb (A15949)

Western blot analysis of various lysates using TPRG1L Rabbit pAb (A15949) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product nameTPRG1L Rabbit pAb
Catalog No.A15949
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable identical protein binding activity. Predicted to be involved in regulation of glutamatergic synaptic transmission. Located in extracellular exosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human TPRG1L (NP_877429.2).
SequenceMLQLRDSVDSAGTSPTAVLAAGEEVGAGGGPGGGRPGAGTPLRQTLWPLSIHDPTRRARVKEYFVFRPGSIEQAVEEIRVVVRPVEDGEI
Gene ID
Swiss Prot
SynonymsTPRGL; mover; FAM79A; SVAP30; h-mover; TPRG1L
Calculated MW30kDa
Observed MW30kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain, Rat brain
Cellular locationCytoplasmic vesicle, secretory vesicle, synaptic vesicle
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

Documents

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    ABclonal:Western blot - TPRG1L Rabbit pAb (A15949)}

    Western blot - TPRG1L Rabbit pAb (A15949)

    Western blot analysis of various lysates using TPRG1L Rabbit pAb (A15949) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A15949? Please let us know so that we can cite the reference in this datasheet.