제품 > 항체 > 다중클론항체(pAb)

TRAPPC10 Rabbit pAb (A6777)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - TRAPPC10 Rabbit pAb (A6777)

Western blot analysis of extracts of various cell lines, using TRAPPC10 antibody (A6777) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15min.

Overview

Product nameTRAPPC10 Rabbit pAb
Catalog No.A6777
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transmembrane protein found in the cis-Golgi complex. The encoded protein is part of the multisubunit transport protein particle (TRAPP) complex and may be involved in vesicular transport from the endoplasmic reticulum to the Golgi. Mutations in this gene could be responsible for the Unverricht-Lundborg type of progressive myoclonus epilepsy, or for autoimmune polyglandular disease type 1.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TRAPPC10 (NP_003265.3).
SequenceMDASEEPLPPVIYTMENKPIVTCAGDQNLFTSVYPTLSQQLPREPMEWRRSYGRAPKMIHLESNFVQFKEELLPKEGNKALLTFPFLHIYWTECCDTEVYKATVKDDLTKWQNVLKAHSSVDWLIVIVENDAKKKNKTNILPRTSIVDKIRNDFCNKQSDRCVVLSDPLKDSSRTQESWNAFLTKLRTLLLMSFTKNLGKFEDDMRTLREKRTEPGWSFCEYFMVQEELAFVFEMLQQFEDALVQYDELDALFSQYVVNF
Gene ID
Swiss Prot
SynonymsEHOC1; GT334; TMEM1; TRS30; EHOC-1; TRS130; NEDMISS
Calculated MW142kDa
Observed MW112kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse kidney, Rat lung
Cellular locationGolgi apparatus, cis-Golgi network
Customer validation

WB(Homo sapiens)

    ABclonal:Western blot - TRAPPC10 Rabbit pAb (A6777)}

    Western blot - TRAPPC10 Rabbit pAb (A6777)

    Western blot analysis of extracts of various cell lines, using TRAPPC10 antibody (A6777) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15min.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)