제품 > 항체 > 다중클론항체(pAb)

TRIM21/SS-A Rabbit pAb (A13547)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using TRIM21/SS-A Rabbit pAb (A13547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - TRIM21/SS-A Rabbit pAb (A13547)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using TRIM21/SS-A Rabbit pAb (A13547) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat kidney using TRIM21/SS-A Rabbit pAb (A13547) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TRIM21/SS-A Rabbit pAb (A13547)

Immunofluorescence analysis of THP-1 cells using TRIM21/SS-A Rabbit pAb(A13547) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - TRIM21/SS-A Rabbit pAb (A13547)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg TRIM21/SS-A Rabbit antibody (A13547). Western blot was performed from the immunoprecipitate using TRIM21/SS-A Rabbit antibody (A13547) at a dilution of 1:1000.

Overview

Product name	TRIM21/SS-A Rabbit pAb
Catalog No.	A13547
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 86-334 of human TRIM21/SS-A (NP_003132.2).
Sequence	GTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGA
Gene ID
Swiss Prot
Synonyms	SSA; RO52; SSA1; RNF81; Ro/SSA; TRIM21/SS-A
Calculated MW	54kDa
Observed MW

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	A-549, Jurkat, HeLa
Cellular location	Cytoplasm, Cytoplasmic vesicle, Nucleus, P-body, autophagosome
Customer validation	WB(Mus musculus) IHC(Mus musculus)