제품 > 항체 > 다중클론항체(pAb)

TRIM4 Rabbit pAb (A15922)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - TRIM4 Rabbit pAb (A15922)

Western blot analysis of extracts of various cell lines, using TRIM4 antibody (A15922) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - TRIM4 Rabbit pAb (A15922)

Immunofluorescence analysis of U2OS cells using TRIM4 Rabbit pAb (A15922) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameTRIM4 Rabbit pAb
Catalog No.A15922
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternatively spliced transcript variants that encode different isoforms have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TRIM4 (NP_148977.2).
SequenceMEAEDIQEELTCPICLDYFQDPVSIECGHNFCRGCLHRNWAPGGGPFPCPECRHPSAPAALRPNWALARLTEKTQRRRLGPVPPGLCGRHWEPLRLFCEDDQRPVCLVCRESQEHQTHAMAPIDEAFESYRTGNFDIHVDEWKRRLIRLLLYHFKQEEKLLKSQRNLVAKMKKVMHLQDVEVKNATQWKDKIKSQRMRISTEFSKLHNFLVEEEDLFLQRLNKEEEETKKKLNENTLKLNQTIASLKKLILEVGEKSQAPTLELLQNPKEVLTRSEIQDV
Gene ID
Swiss Prot
SynonymsRNF87
Calculated MW34kDa/54kDa/57kDa
Observed MW50-60kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, HepG2, MCF7
Cellular locationCytoplasm
Customer validation

Co-IP(Homo sapiens)

WB(Homo sapiens)

IF(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - TRIM4 Rabbit pAb (A15922)}

    Western blot - TRIM4 Rabbit pAb (A15922)

    Western blot analysis of extracts of various cell lines, using TRIM4 antibody (A15922) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - TRIM4 Rabbit pAb (A15922)}

    Immunofluorescence - TRIM4 Rabbit pAb (A15922)

    Immunofluorescence analysis of U2OS cells using TRIM4 Rabbit pAb (A15922) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A15922? Please let us know so that we can cite the reference in this datasheet.