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TRPV1 Rabbit pAb (A8564)

Publications (16) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TRPV1 Rabbit pAb (A8564)

Western blot analysis of lysates from Mouse brain, using TRPV1 Rabbit pAb (A8564) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - TRPV1 Rabbit pAb (A8564)

Immunofluorescence analysis of NIH/3T3 cells using TRPV1 Rabbit pAb (A8564) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameTRPV1 Rabbit pAb
Catalog No.A8564
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 725-839 of human TRPV1 (NP_061197.4).
SequenceKLLQVGYTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGVKRTLSFSLRSSRVSGRHWKNFALVPLLREASARDRQSAQPEEVYLRQFSGSLKPEDAEVFKSPAASGEK
Gene ID
Swiss Prot
SynonymsVR1; TRPV1
Calculated MW95kDa
Observed MW102kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain
Cellular locationCell junction, Cell membrane, Cell projection, Multi-pass membrane protein, dendritic spine membrane, postsynaptic cell membrane, synapse.
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens)

IF(Mus musculus, Homo sapiens, Canis familiaris)

IHC(Mus musculus)

IF(Homo sapiens, Mus musculus)

WB(Canis familiaris)

Documents

Certificate of Compliance

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    ABclonal:Western blot - TRPV1 Rabbit pAb (A8564)}

    Western blot - TRPV1 Rabbit pAb (A8564)

    Western blot analysis of lysates from Mouse brain, using TRPV1 Rabbit pAb (A8564) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - TRPV1 Rabbit pAb (A8564)}

    Immunofluorescence - TRPV1 Rabbit pAb (A8564)

    Immunofluorescence analysis of NIH/3T3 cells using TRPV1 Rabbit pAb (A8564) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8564? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)