제품 > 항체 > 다중클론항체(pAb)

TRPV5 Rabbit pAb (A6473)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using TRPV5 Rabbit pAb (A6473) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - TRPV5 Rabbit pAb (A6473)

Immunohistochemistry analysis of paraffin-embedded human kidney using TRPV5 Rabbit pAb (A6473) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	TRPV5 Rabbit pAb
Catalog No.	A6473
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 655-725 of human TRPV5 (NP_062815.3).
Sequence	DKEDDQEHPSEKQPSGAESGTLARASLALPTSSLSRTASQSSSHRGWEILRQNTLGHLNLGLNLSEGDGEE
Gene ID
Swiss Prot
Synonyms	CAT2; ECAC1; OTRPC3
Calculated MW	83kDa
Observed MW	75kDa/85-100kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HepG2, 293F, Mouse kidney, Mouse heart, Rat heart, Rat kidney
Cellular location	Apical cell membrane, Multi-pass membrane protein
Customer validation	WB(Homo sapiens)