제품 > 항체 > 다중클론항체(pAb)

TSLP Rabbit pAb (A13134)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TSLP Rabbit pAb (A13134)

Western blot analysis of various lysates using TSLP Rabbit pAb (A13134) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - TSLP Rabbit pAb (A13134)

Immunofluorescence analysis of C6 cells using TSLP Rabbit pAb (A13134) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TSLP Rabbit pAb (A13134)

Immunofluorescence analysis of L929 cells using TSLP Rabbit pAb (A13134) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TSLP Rabbit pAb (A13134)

Immunofluorescence analysis of U-2 OS cells using TSLP Rabbit pAb (A13134) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameTSLP Rabbit pAb
Catalog No.A13134
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. In addition, the shorter (predominant) isoform is an antimicrobial protein, displaying antibacterial and antifungal activity against B. cereus, E. coli, E. faecalis, S. mitis, S. epidermidis, and C. albicans. Alternative splicing of this gene results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 29-159 of human TSLP (NP_149024.1).
SequenceYDFTNCDFEKIKAAYLSTISKDLITYMSGTKSTEFNNTVSCSNRPHCLTEIQSLTFNPTAGCASLAKEMFAMKTKAALAIWCPGYSETQINATQAMKKRRKRKVTTNKCLEQVSQLQGLWRRFNRPLLKQQ
Gene ID
Swiss Prot
SynonymsTSLP
Calculated MW18kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, Rat brain
Cellular locationSecreted.
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - TSLP Rabbit pAb (A13134)}

Western blot - TSLP Rabbit pAb (A13134)

Western blot analysis of various lysates using TSLP Rabbit pAb (A13134) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - TSLP Rabbit pAb (A13134)}

Immunofluorescence - TSLP Rabbit pAb (A13134)

Immunofluorescence analysis of C6 cells using TSLP Rabbit pAb (A13134) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TSLP Rabbit pAb (A13134)}

Immunofluorescence - TSLP Rabbit pAb (A13134)

Immunofluorescence analysis of L929 cells using TSLP Rabbit pAb (A13134) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TSLP Rabbit pAb (A13134)}

Immunofluorescence - TSLP Rabbit pAb (A13134)

Immunofluorescence analysis of U-2 OS cells using TSLP Rabbit pAb (A13134) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A13134? Please let us know so that we can cite the reference in this datasheet.

ELISA 키트 (1)

재조합 단백질 (2)

항체 (1)

제품 (4)

Secondary Antibodies (26)