제품 > 항체 > 다중클론항체(pAb)

TTBK2 Rabbit pAb (A7609)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of lysates from rat brain, using TTBK2 Rabbit pAb (A7609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TTBK2 Rabbit pAb (A7609)

Immunohistochemistry analysis of paraffin-embedded Human stomach using TTBK2 Rabbit pAb (A7609) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product name	TTBK2 Rabbit pAb
Catalog No.	A7609
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a serine-threonine kinase that putatively phosphorylates tau and tubulin proteins. Mutations in this gene cause spinocerebellar ataxia type 11 (SCA11); a neurodegenerative disease characterized by progressive ataxia and atrophy of the cerebellum and brainstem.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1185-1244 of human TTBK2 (NP_775771.3).
Sequence	LGRSKSPPSHSGSSSSRRSCQQEHCKPSKNGLKGSGSLHHHSASTKTPQGKSKPASKLSR
Gene ID
Swiss Prot
Synonyms	TTBK; SCA11; TTBK2
Calculated MW	137kDa
Observed MW	137kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Rat brain
Cellular location	Cell projection, Cytoplasm, Nucleus, centriole, centrosome, cilium, cilium basal body, cytoskeleton, cytosol, microtubule organizing center