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Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782)

Western blot analysis of extracts of various cell lines, using Thioredoxin 2 (Trx2/Thioredoxin 2 (Trx2/TXN2)) antibody (A6782) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782)

Immunofluorescence analysis of NIH/3T3 cells using Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782)

Immunofluorescence analysis of U2OS cells using Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameThioredoxin 2 (Trx2/TXN2) Rabbit pAb
Catalog No.A6782
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human Thioredoxin 2 (Trx2/Thioredoxin 2 (Trx2/TXN2)) (NP_036605.2).
SequenceMAQRLLLRRFLASVISRKPSQGQWPPLTSRALQTPQCSPGGLTVTPNPARTIYTTRISLTTFNIQDGPDFQDRVVNSETPVVVDFHAQWCGPCKILGPRLEKMVAKQHGKVVMAKVDIDDHTDLAIEYEVSAVPTVLAMKNGDVVDKFVGIKDEDQLEAFLKKLIG
Gene ID
Swiss Prot
SynonymsTXN; MTRX; TRX2; MT-TRX; COXPD29
Calculated MW18kDa
Observed MW13kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRaji, 293T, SW620, THP-1
Cellular locationMitochondrion

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ABclonal:Western blot - Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782)}

Western blot - Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782)

Western blot analysis of extracts of various cell lines, using Thioredoxin 2 (Trx2/Thioredoxin 2 (Trx2/TXN2)) antibody (A6782) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782)}

Immunofluorescence - Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782)

Immunofluorescence analysis of NIH/3T3 cells using Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782)}

Immunofluorescence - Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782)

Immunofluorescence analysis of U2OS cells using Thioredoxin 2 (Trx2/TXN2) Rabbit pAb (A6782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

재조합 단백질 (1)

Secondary Antibodies (26)