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Thymidine Kinase 1 Rabbit pAb (A5612)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Thymidine Kinase 1 Rabbit pAb (A5612)

Western blot analysis of various lysates using Thymidine Kinase 1 Rabbit pAb (A5612) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of HeLa cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of MCF7 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of NIH/3T3 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

Immunofluorescence analysis of PC-12 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameThymidine Kinase 1 Rabbit pAb
Catalog No.A5612
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 15-194 of human Thymidine Kinase 1 (NP_003249.3).
SequenceSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKAS
Gene ID
Swiss Prot
SynonymsTK2; Thymidine Kinase 1
Calculated MW25kDa
Observed MW26kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, Jurkat, Mouse liver, Mouse spleen, Mouse stomach, Rat liver, Rat spleen, Rat stomach
Cellular locationCytoplasm.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Thymidine Kinase 1 Rabbit pAb (A5612)}

    Western blot - Thymidine Kinase 1 Rabbit pAb (A5612)

    Western blot analysis of various lysates using Thymidine Kinase 1 Rabbit pAb (A5612) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - Thymidine Kinase 1 Rabbit pAb (A5612)}

    Immunohistochemistry - Thymidine Kinase 1 Rabbit pAb (A5612)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)}

    Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

    Immunofluorescence analysis of HeLa cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)}

    Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

    Immunofluorescence analysis of MCF7 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)}

    Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

    Immunofluorescence analysis of NIH/3T3 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)}

    Immunofluorescence - Thymidine Kinase 1 Rabbit pAb (A5612)

    Immunofluorescence analysis of PC-12 cells using Thymidine Kinase 1 Rabbit pAb (A5612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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