제품 > 항체 > 다중클론항체(pAb)

Timm29 Rabbit pAb (A20812)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Timm29 Rabbit pAb (A20812)

Western blot analysis of various lysates using Timm29 Rabbit pAb (A20812) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

ABclonal:Immunoprecipitation - Timm29 Rabbit pAb (A20812)

Immunoprecipitation analysis of 300 μg extracts of A-549 cells using 3 μg Timm29 antibody (A20812). Western blot was performed from the immunoprecipitate using Timm29 antibody (A20812) at a dilution of 1:1000.

Overview

Product nameTimm29 Rabbit pAb
Catalog No.A20812
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable protein transporter activity. Predicted to be involved in protein insertion into mitochondrial inner membrane. Predicted to act upstream of or within protein transport. Predicted to be located in mitochondrial inner membrane and mitochondrial intermembrane space. Predicted to be part of TIM22 mitochondrial import inner membrane insertion complex. Orthologous to human TIMM29 (translocase of inner mitochondrial membrane 29).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 157-266 of mouse Timm29 (NP_848734.1).
SequenceYLQPRWVDFPGRILDVGFVGRWWILQNRMHDCDINDDEFLHLPAHLRVVAPHQLHSEANERLFEEKYKPIILTDDQVDQALWEEQVLQKERKDRLALSEADSLVQSDVSR
Gene ID
Swiss Prot
SynonymsTIM29; 1810026J23Rik; Timm29
Calculated MW29kDa
Observed MW29kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samples293T, A-549, PC-3, Mouse liver
Cellular locationmitochondrial inner membrane, mitochondrial intermembrane space

Documents

Certificate of Compliance

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    ABclonal:Western blot - Timm29 Rabbit pAb (A20812)}

    Western blot - Timm29 Rabbit pAb (A20812)

    Western blot analysis of various lysates using Timm29 Rabbit pAb (A20812) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 120s.
    ABclonal:Immunoprecipitation - Timm29 Rabbit pAb (A20812)}

    Immunoprecipitation - Timm29 Rabbit pAb (A20812)

    Immunoprecipitation analysis of 300 μg extracts of A-549 cells using 3 μg Timm29 antibody (A20812). Western blot was performed from the immunoprecipitate using Timm29 antibody (A20812) at a dilution of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.