제품 > 항체 > 다중클론항체(pAb)

Topors Rabbit pAb (A18526)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - Topors Rabbit pAb (A18526)

Western blot analysis of various lysates using Topors Rabbit pAb (A18526) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product nameTopors Rabbit pAb
Catalog No.A18526
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable several functions, including DNA topoisomerase binding activity; antigen binding activity; and ubiquitin-like protein transferase activity. Acts upstream of or within intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; positive regulation of transcription, DNA-templated; and regulation of cell population proliferation. Located in ciliary basal body; nucleus; and photoreceptor connecting cilium. Is expressed in skin. Human ortholog(s) of this gene implicated in retinitis pigmentosa 31. Orthologous to human TOPORS (TOP1 binding arginine/serine rich protein, E3 ubiquitin ligase).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of mouse Topors (NP_598858.2).
SequencePDSKCPICLDRFDNVSYLDRCLHKFCFRCVQEWSKNKAECPLCKQPFDSIFHSVRAEDDFKEYVLRPSYNGSFTNPEVRRFRYRTTMTRERSASLYSPSST
Gene ID
Swiss Prot
SynonymsLUN; TP53BPL; p53BP3/LUN; Topors
Calculated MW117kDa
Observed MW119kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse liver, Mouse lung, Mouse kideny, Rat kidney
Cellular locationcentriole, ciliary basal body, gamma-tubulin complex, nuclear speck, nucleoplasm, nucleus, PML body, spindle pole

Documents

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    ABclonal:Western blot - Topors Rabbit pAb (A18526)}

    Western blot - Topors Rabbit pAb (A18526)

    Western blot analysis of various lysates using Topors Rabbit pAb (A18526) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.

    * For research use only. Not for therapeutic or diagnostic purposes.