Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of various lysates using Transglutaminase 2 (TGM2) Rabbit mAb (A21184) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Transglutaminase 2 (TGM2) Rabbit mAb (A21184) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Transglutaminase 2 (TGM2) Rabbit mAb (A21184) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using Transglutaminase 2 (TGM2) Rabbit mAb (A21184) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung tissue using Transglutaminase 2 (TGM2) Rabbit mAb (A21184) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Confocal imaging of HeLa cells using Transglutaminase 2 (TGM2) Rabbit mAb (A21184, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
| Product name | Transglutaminase 2 (TGM2) Rabbit mAb |
|---|---|
| Catalog No. | A21184 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC52843 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 438-687 of human Transglutaminase 2 (TGM2) (NP_004604.2). |
|---|---|
| Sequence | DITHTYKYPEGSSEEREAFTRANHLNKLAEKEETGMAMRIRVGQSMNMGSDFDVFAHITNNTAEEYVCRLLLCARTVSYNGILGPECGTKYLLNLNLEPFSEKSVPLCILYEKYRDCLTESNLIKVRALLVEPVINSYLLAERDLYLENPEIKIRILGEPKQKRKLVAEVSLQNPLPVALEGCTFTVEGAGLTEEQKTVEIPDPVEAGEEVKVRMDLLPLHMGLHKLVVNFESDKLKAVKGFRNVIIGPA |
| Gene ID | |
| Swiss Prot | |
| Synonyms | TGC; tTG; G(h); hTG2; TG(C); Transglutaminase 2 (TGM2) |
| Calculated MW | 77kDa |
| Observed MW | 77kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | A549, K-562, Hep G2 |
| Cellular location | cytosol, endoplasmic reticulum, extracellular exosome, focal adhesion, mitochondrion, nucleus, perinuclear region of cytoplasm, plasma membrane. |
| Customer validation | (A549) IF(Homo sapiens) WB(Homo sapiens) |
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