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Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A22440)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A22440)

Western blot analysis of lysates from U-937 cells using Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A22440) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A22440)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A22440) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameTryptophanyl-tRNA synthetase 1 Rabbit mAb
Catalog No.A22440
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2687
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 372-471 of human Tryptophanyl-tRNA synthetase 1 (P23381).
SequenceVNKHAFSGGRDTIEEHRQFGGNCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKEVTDEIVKEFMTPRKLSFDFQ
Gene ID
Swiss Prot
SynonymsHMN9; WARS; IFI53; IFP53; GAMMA-2; Tryptophanyl-tRNA synthetase 1
Calculated MW53kDa
Observed MW53kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-937
Cellular locationCytoplasm.

Documents

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    ABclonal:Western blot - Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A22440)}

    Western blot - Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A22440)

    Western blot analysis of lysates from U-937 cells using Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A22440) at 1:1000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A22440)}

    Immunohistochemistry - Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A22440)

    Immunohistochemistry analysis of paraffin-embedded Human placenta using Tryptophanyl-tRNA synthetase 1 Rabbit mAb (A22440) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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