제품 > 항체 > Tag & 로딩 컨트롤 항체

β-Tubulin Mouse mAb (AC010)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using β-Tubulin Mouse mAb (AC010) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Review

Overview

Product name	β-Tubulin Mouse mAb
Catalog No.	AC010
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG1
CloneNo.	AMC0497

Background

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Immunogen information

Immunogen	Recombinant protein of human TUBB
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56; β-Tubulin
Calculated MW	50kDa
Observed MW	55kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:6000 IHC-P 1:200 - 1:500 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	U-87MG, Mouse testis, Mouse brain, Rat spinal cord, Rat brain
Cellular location	Cytoplasm, cytoskeleton.
Customer validation	(Mouse mature neuron cells) WB(Homo sapiens, Arabidopsis thaliana, Mus musculus, Mus musculus, Rattus norvegicus) IP(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

β-Tubulin Mouse mAb images

Western blot - β-Tubulin Mouse mAb (AC010)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for AC010

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Product Protocols

References for β-Tubulin Mouse mAb (AC010)

Product:

β-Tubulin Mouse mAb

Journal:

Reproduction

Application:

IF:

3.7

Species:

Homo sapiens

PMID:

30780128

Title:

H19 regulates trophoblastic spheroid adhesion by competitively binding to let-7.

References for β-Tubulin Mouse mAb (AC010)

Product:

β-Tubulin Mouse mAb

Journal:

Nature

Application:

IF:

50.5

Species:

Arabidopsis thaliana

PMID:

32376953

Title:

Plant 22-nt siRNAs mediate translational repression and stress adaptation

References for β-Tubulin Mouse mAb (AC010)

Product:

β-Tubulin Mouse mAb

Journal:

Life Sciences

Application:

IF:

5.2

Species:

Mus musculus

PMID:

33157091

Title:

Taxifolin improves disorders of glucose metabolism and water-salt metabolism in kidney via PI3K/AKT signaling pathway in metabolic syndrome rats

References for β-Tubulin Mouse mAb (AC010)

Product:

β-Tubulin Mouse mAb

Journal:

NPJ Genom Med

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

33397963

Title:

Biallelic SORD pathogenic variants cause Chinese patients with distal hereditary motor neuropathy

References for β-Tubulin Mouse mAb (AC010)

Product:

β-Tubulin Mouse mAb

Journal:

Cell reports

Application:

IF:

7.5

Species:

Homo sapiens

PMID:

35476980

Title:

RPAP2 regulates a transcription initiation checkpoint by inhibiting assembly of pre-initiation complex

References for β-Tubulin Mouse mAb (AC010)

Product:

β-Tubulin Mouse mAb

Journal:

Oncology Reports

Application:

IF:

3.8

Species:

Homo sapiens, Mus musculus

PMID:

31894280

Title:

Dexamethasone induces aberrant macrophage immune function and apoptosis

References for β-Tubulin Mouse mAb (AC010)

Product:

β-Tubulin Mouse mAb

Journal:

Nature chemical biology

Application:

WB, IP

IF:

12.9

Species:

Homo sapiens

PMID:

36635566

Title:

SIRT1 deacetylates WEE1 and sensitizes cancer cells to WEE1 inhibition

References for β-Tubulin Mouse mAb (AC010)

Product:

β-Tubulin Mouse mAb

Journal:

Human cell

Application:

IF:

3.4

Species:

Homo sapiens

PMID:

38935235

Title:

KIN17 functions in DNA damage repair and chemosensitivity by modulating RAD51 in hepatocellular carcinoma

References for β-Tubulin Mouse mAb (AC010)

Product:

β-Tubulin Mouse mAb

Journal:

Free radical biology & medicine

Application:

IF:

7.1

Species:

Mus musculus,Rattus norvegicus

PMID:

39827920

Title:

Mitochondrial targeted drug Dichloroacetate protects against sulfur mustard-induced neurotoxicity via the PDK/PDH axis and Akt/Nrf2 pathway

Publishing research using AC010? Please let us know so that we can cite the reference in this datasheet.

항체

분자 생물학 시약

ELISA 키트

ELISA 항체 pair

NGS 라이브러리 제작 키트

분자 진단 원료

재조합 단백질

실험용품

생화학 시약

Popular Pathways

연구분야

제품 > 항체 > Tag & 로딩 컨트롤 항체

β-Tubulin Mouse mAb (AC010)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

β-Tubulin Mouse mAb images

Submit an review for AC010

Reviews List

Product Protocols

항체 (5)

Secondary Antibodies (22)

Discontinued