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U1A/SNRPA Rabbit pAb (A6410)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - U1A/SNRPA Rabbit pAb (A6410)

Western blot analysis of various lysates using U1A/SNRPA Rabbit pAb (A6410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - U1A/SNRPA Rabbit pAb (A6410)

Immunohistochemistry analysis of paraffin-embedded Rat heart using U1A/SNRPA Rabbit pAb (A6410) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - U1A/SNRPA Rabbit pAb (A6410)

Immunohistochemistry analysis of paraffin-embedded Human adenomyosis using U1A/SNRPA Rabbit pAb (A6410) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - U1A/SNRPA Rabbit pAb (A6410)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using U1A/SNRPA Rabbit pAb (A6410) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - U1A/SNRPA Rabbit pAb (A6410)

Immunofluorescence analysis of HeLa cells using U1A/SNRPA Rabbit pAb (A6410).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Overview

Product nameU1A/SNRPA Rabbit pAb
Catalog No.A6410
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human U1A/U1A/SNRPA (NP_004587.1).
SequenceMAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAMKISFAKK
Gene ID
Swiss Prot
SynonymsU1A; Mud1; U1-A; U1A/SNRPA
Calculated MW31kDa
Observed MW31kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, 293T, A-431, MCF7, Mouse brain, Mouse testis, Mouse thymus, Rat brain
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - U1A/SNRPA Rabbit pAb (A6410)}

    Western blot - U1A/SNRPA Rabbit pAb (A6410)

    Western blot analysis of various lysates using U1A/SNRPA Rabbit pAb (A6410) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - U1A/SNRPA Rabbit pAb (A6410)}

    Immunohistochemistry - U1A/SNRPA Rabbit pAb (A6410)

    Immunohistochemistry analysis of paraffin-embedded Rat heart using U1A/SNRPA Rabbit pAb (A6410) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - U1A/SNRPA Rabbit pAb (A6410)}

    Immunohistochemistry - U1A/SNRPA Rabbit pAb (A6410)

    Immunohistochemistry analysis of paraffin-embedded Human adenomyosis using U1A/SNRPA Rabbit pAb (A6410) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - U1A/SNRPA Rabbit pAb (A6410)}

    Immunohistochemistry - U1A/SNRPA Rabbit pAb (A6410)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using U1A/SNRPA Rabbit pAb (A6410) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - U1A/SNRPA Rabbit pAb (A6410)}

    Immunofluorescence - U1A/SNRPA Rabbit pAb (A6410)

    Immunofluorescence analysis of HeLa cells using U1A/SNRPA Rabbit pAb (A6410).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A6410? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)