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U2AF2/U2AF65 Rabbit mAb (A4552)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - U2AF2/U2AF65 Rabbit mAb (A4552)

Western blot analysis of various lysates using U2AF2/U2AF2/U2AF65 Rabbit mAb (A4552) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - U2AF2/U2AF65 Rabbit mAb (A4552)

Western blot analysis of various lysates using U2AF2/U2AF2/U2AF65 Rabbit mAb (A4552) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunoprecipitation - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg U2AF2/U2AF65 antibody (A4552). Western blot was performed from the immunoprecipitate using U2AF2/U2AF65 antibody (A4552) at a dilution of 1:1000.

Overview

Product nameU2AF2/U2AF65 Rabbit mAb
Catalog No.A4552
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1029
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 376-475 of human U2AF2/U2AF2/U2AF65 (P26368).
SequenceEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW
Gene ID
Swiss Prot
SynonymsU2AF65; U2AF2/U2AF65
Calculated MW54kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesHeLa, HT-29, Mouse lung, Mouse brain, Rat brain
Cellular locationNucleus.

Documents

Certificate of Compliance

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ABclonal:Western blot - U2AF2/U2AF65 Rabbit mAb (A4552)}

Western blot - U2AF2/U2AF65 Rabbit mAb (A4552)

Western blot analysis of various lysates using U2AF2/U2AF2/U2AF65 Rabbit mAb (A4552) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - U2AF2/U2AF65 Rabbit mAb (A4552)}

Western blot - U2AF2/U2AF65 Rabbit mAb (A4552)

Western blot analysis of various lysates using U2AF2/U2AF2/U2AF65 Rabbit mAb (A4552) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)}

Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)}

Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)}

Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)}

Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)}

Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)}

Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)}

Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)}

Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)}

Immunohistochemistry - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using U2AF2/U2AF65 Rabbit mAb (A4552) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunoprecipitation - U2AF2/U2AF65 Rabbit mAb (A4552)}

Immunoprecipitation - U2AF2/U2AF65 Rabbit mAb (A4552)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg U2AF2/U2AF65 antibody (A4552). Western blot was performed from the immunoprecipitate using U2AF2/U2AF65 antibody (A4552) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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