제품 > 항체 > 다중클론항체(pAb)

UACA Rabbit pAb (A9713)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UACA Rabbit pAb (A9713)

Western blot analysis of extracts of various cell lines, using UACA antibody (A9713) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameUACA Rabbit pAb
Catalog No.A9713
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that contains ankyrin repeats and coiled coil domains and likely plays a role in apoptosis. Studies in rodents have implicated the encoded protein in the stimulation of apoptosis and the regulation of mammary gland involution, in which the mammary gland returns to its pre-pregnant state. This protein has also been proposed to negatively regulate apoptosis based on experiments in human cell lines in which the protein was shown to interact with PRKC apoptosis WT1 regulator protein, also known as PAR-4, and inhibit translocation of the PAR-4 receptor. Autoantibodies to this protein have been identified in human patients with panuveitis and Graves' disease. Differential expression of this gene has been observed in various human cancers.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human UACA (NP_060473.2).
SequenceMKSLKSRLRRQDVPGPASSGAAAASAHAADWNKYDDRLMKAAERGDVEKVTSILAKKGVNPGKLDVEGRSVFHVVTSKGNLECLNAILIH
Gene ID
Swiss Prot
SynonymsNUCLING
Calculated MW163kDa
Observed MW162kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, 293T, HT-1080, SKOV3
Cellular locationCytoplasm, Nucleus, cytoskeleton

Documents

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    ABclonal:Western blot - UACA Rabbit pAb (A9713)}

    Western blot - UACA Rabbit pAb (A9713)

    Western blot analysis of extracts of various cell lines, using UACA antibody (A9713) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)