제품 > 항체 > 단일클론항체(mAb)

UAP1 Rabbit mAb (A8662)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UAP1 Rabbit mAb (A8662)

Western blot analysis of extracts of various cell lines, using UAP1 Rabbit mAb (A8662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - UAP1 Rabbit mAb (A8662)

Immunofluorescence analysis of C6 cells using UAP1 Rabbit mAb (A8662) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UAP1 Rabbit mAb (A8662)

Immunofluorescence analysis of U-2 OS cells using UAP1 Rabbit mAb (A8662) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameUAP1 Rabbit mAb
Catalog No.A8662
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1769
Enables identical protein binding activity. Predicted to be involved in UDP-N-acetylglucosamine biosynthetic process. Located in cytosol; nucleoplasm; and plasma membrane.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 400-500 of human UAP1 (Q16222).
SequenceEDEFSPLKNADSQNGKDNPTTARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRSATNGKSETITADVNHNLKDANDVPIQCEISPLISYAGEGLESYVAD
Gene ID
Swiss Prot
SynonymsAGX; AGX1; AGX2; SPAG2
Calculated MW59kDa
Observed MW59kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2, DU 145, Jurkat, Mouse brain, Rat brain
Cellular locationCytoplasm

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - UAP1 Rabbit mAb (A8662)}

    Western blot - UAP1 Rabbit mAb (A8662)

    Western blot analysis of extracts of various cell lines, using UAP1 Rabbit mAb (A8662) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - UAP1 Rabbit mAb (A8662)}

    Immunofluorescence - UAP1 Rabbit mAb (A8662)

    Immunofluorescence analysis of C6 cells using UAP1 Rabbit mAb (A8662) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - UAP1 Rabbit mAb (A8662)}

    Immunofluorescence - UAP1 Rabbit mAb (A8662)

    Immunofluorescence analysis of U-2 OS cells using UAP1 Rabbit mAb (A8662) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)