제품 > 항체 > 다중클론항체(pAb)

UBA1 Rabbit pAb (A12359)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UBA1 Rabbit pAb (A12359)

Western blot analysis of various lysates using UBA1 Rabbit pAb (A12359) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - UBA1 Rabbit pAb (A12359)

Immunohistochemistry analysis of paraffin-embedded Rat liver using UBA1 Rabbit pAb (A12359) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - UBA1 Rabbit pAb (A12359)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using UBA1 Rabbit pAb (A12359) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - UBA1 Rabbit pAb (A12359)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using UBA1 Rabbit pAb (A12359) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - UBA1 Rabbit pAb (A12359)

Immunofluorescence analysis of HeLa cells using UBA1 Rabbit pAb (A12359) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameUBA1 Rabbit pAb
Catalog No.A12359
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human UBA1 (NP_695012.1).
SequenceKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGT
Gene ID
Swiss Prot
SynonymsA1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; VEXAS; CFAP124; UBA1
Calculated MW118kDa
Observed MW121kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-251MG, Rat spleen
Cellular locationCytoplasm, Mitochondrion, Nucleus, Nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - UBA1 Rabbit pAb (A12359)}

Western blot - UBA1 Rabbit pAb (A12359)

Western blot analysis of various lysates using UBA1 Rabbit pAb (A12359) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - UBA1 Rabbit pAb (A12359)}

Immunohistochemistry - UBA1 Rabbit pAb (A12359)

Immunohistochemistry analysis of paraffin-embedded Rat liver using UBA1 Rabbit pAb (A12359) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - UBA1 Rabbit pAb (A12359)}

Immunohistochemistry - UBA1 Rabbit pAb (A12359)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using UBA1 Rabbit pAb (A12359) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - UBA1 Rabbit pAb (A12359)}

Immunohistochemistry - UBA1 Rabbit pAb (A12359)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using UBA1 Rabbit pAb (A12359) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - UBA1 Rabbit pAb (A12359)}

Immunofluorescence - UBA1 Rabbit pAb (A12359)

Immunofluorescence analysis of HeLa cells using UBA1 Rabbit pAb (A12359) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)