제품 > 항체 > 다중클론항체(pAb)

UBA2 Rabbit pAb (A17342)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - UBA2 Rabbit pAb (A17342)

Western blot analysis of lysates from Rat lung, using UBA2 Rabbit pAb (A17342) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameUBA2 Rabbit pAb
Catalog No.A17342
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 60-160 of human UBA2 (NP_005490.1).
SequenceQFLFQKKHVGRSKAQVAKESVLQFYPKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESGTAGYLGQVTTIKKGVTECYE
Gene ID
Swiss Prot
SynonymsARX; SAE2; ACCES; HRIHFB2115; UBA2
Calculated MW71kDa
Observed MW90kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat lung
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - UBA2 Rabbit pAb (A17342)}

Western blot - UBA2 Rabbit pAb (A17342)

Western blot analysis of lysates from Rat lung, using UBA2 Rabbit pAb (A17342) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)