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UBE2A Rabbit pAb (A12528)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - UBE2A Rabbit pAb (A12528)

Western blot analysis of extracts of mouse kidney, using UBE2A antibody (A12528) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - UBE2A Rabbit pAb (A12528)

Immunofluorescence analysis of U2OS cells using UBE2A antibody (A12528) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameUBE2A Rabbit pAb
Catalog No.A12528
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2).
SequenceMSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC
Gene ID
Swiss Prot
SynonymsUBC2; HHR6A; MRXSN; RAD6A; MRXS30
Calculated MW17kDa
Observed MW17kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse kidney
Cellular locationcytosol, nucleoplasm

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ABclonal:Western blot - UBE2A Rabbit pAb (A12528)}

Western blot - UBE2A Rabbit pAb (A12528)

Western blot analysis of extracts of mouse kidney, using UBE2A antibody (A12528) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - UBE2A Rabbit pAb (A12528)}

Immunofluorescence - UBE2A Rabbit pAb (A12528)

Immunofluorescence analysis of U2OS cells using UBE2A antibody (A12528) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)