제품 > 항체 > 다중클론항체(pAb)

UBE2J2 Rabbit pAb (A7316)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UBE2J2 Rabbit pAb (A7316)

Western blot analysis of extracts of various cell lines, using UBE2J2 antibody (A7316) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Overview

Product nameUBE2J2 Rabbit pAb
Catalog No.A7316
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human UBE2J2 (NP_477515.2).
SequenceMSSTSSKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLAVQSLAFNLKDKVFCELFPEVVEEIKQKQKAQDELSSRPQTLPLPDVVPDGETHLVQNGIQLLNGHAPGAVPNLAGLQQANRHHGLLG
Gene ID
Swiss Prot
SynonymsUBC6; NCUBE2; NCUBE-2; PRO2121
Calculated MW29kDa
Observed MW29kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-251MG, HeLa, 293T, Mouse testis, Mouse brain
Cellular locationEndoplasmic reticulum membrane, Single-pass type IV membrane protein

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ABclonal:Western blot - UBE2J2 Rabbit pAb (A7316)}

Western blot - UBE2J2 Rabbit pAb (A7316)

Western blot analysis of extracts of various cell lines, using UBE2J2 antibody (A7316) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)