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UBE2L6 Rabbit pAb (A13670)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UBE2L6 Rabbit pAb (A13670)

Western blot analysis of various lysates, using UBE2L6 Rabbit pAb (A13670) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - UBE2L6 Rabbit pAb (A13670)

Immunofluorescence analysis of HeLa cells using UBE2L6 Rabbit pAb (A13670) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UBE2L6 Rabbit pAb (A13670)

Immunofluorescence analysis of NIH/3T3 cells using UBE2L6 Rabbit pAb (A13670) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameUBE2L6 Rabbit pAb
Catalog No.A13670
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-153 of human UBE2L6 (NP_004214.1).
SequenceKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNAEEFTLRFGVDRPS
Gene ID
Swiss Prot
SynonymsRIG-B; UBCH8; UBE2L6
Calculated MW18kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse lung, Rat kidney
Cellular locationcytosol, nucleoplasm, nucleus.

Documents

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ABclonal:Western blot - UBE2L6 Rabbit pAb (A13670)}

Western blot - UBE2L6 Rabbit pAb (A13670)

Western blot analysis of various lysates, using UBE2L6 Rabbit pAb (A13670) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - UBE2L6 Rabbit pAb (A13670)}

Immunofluorescence - UBE2L6 Rabbit pAb (A13670)

Immunofluorescence analysis of HeLa cells using UBE2L6 Rabbit pAb (A13670) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - UBE2L6 Rabbit pAb (A13670)}

Immunofluorescence - UBE2L6 Rabbit pAb (A13670)

Immunofluorescence analysis of NIH/3T3 cells using UBE2L6 Rabbit pAb (A13670) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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