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UBE2L6 Rabbit mAb (A23917)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UBE2L6 Rabbit mAb (A23917)

Western blot analysis of various lysates using UBE2L6 Rabbit mAb (A23917) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): HeLa.
Exposure time: 1s.

ABclonal:Immunohistochemistry - UBE2L6 Rabbit mAb (A23917)

Immunohistochemistry analysis of paraffin-embedded Human colon using UBE2L6 Rabbit mAb (A23917) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - UBE2L6 Rabbit mAb (A23917)

Immunohistochemistry analysis of paraffin-embedded Rat liver using UBE2L6 Rabbit mAb (A23917) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameUBE2L6 Rabbit mAb
Catalog No.A23917
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC62291
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 54-153 of human UBE2L6 (NP_004214.1).
SequenceRISFPPEYPFKPPMIKFTTKIYHPNVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNAEEFTLRFGVDRPS
Gene ID
Swiss Prot
SynonymsRIG-B; UBCH8; UBE2L6
Calculated MW18kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, Mouse spleen, Rat liver
Cellular locationCytosol, nucleoplasm, nucleus.

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ABclonal:Western blot - UBE2L6 Rabbit mAb (A23917)}

Western blot - UBE2L6 Rabbit mAb (A23917)

Western blot analysis of various lysates using UBE2L6 Rabbit mAb (A23917) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): HeLa.
Exposure time: 1s.
ABclonal:Immunohistochemistry - UBE2L6 Rabbit mAb (A23917)}

Immunohistochemistry - UBE2L6 Rabbit mAb (A23917)

Immunohistochemistry analysis of paraffin-embedded Human colon using UBE2L6 Rabbit mAb (A23917) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - UBE2L6 Rabbit mAb (A23917)}

Immunohistochemistry - UBE2L6 Rabbit mAb (A23917)

Immunohistochemistry analysis of paraffin-embedded Rat liver using UBE2L6 Rabbit mAb (A23917) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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