제품 > 항체 > 다중클론항체(pAb)

ADAM10 Rabbit pAb (A10438)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of RAW264.7, using ADAM10 antibody (A10438) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 20s.

ABclonal:Immunofluorescence - ADAM10 Rabbit pAb (A10438)

Immunofluorescence analysis of HeLa cells using ADAM10 antibody (A10438) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name	ADAM10 Rabbit pAb
Catalog No.	A10438
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 218-433 of human ADAM10 (NP_001101.1).
Sequence	EKNTCQLYIQTDHLFFKYYGTREAVIAQISSHVKAIDTIYQTTDFSGIRNISFMVKRIRINTTADEKDPTNPFRFPNIGVEKFLELNSEQNHDDYCLAYVFTDRDFDDGVLGLAWVGAPSGSSGGICEKSKLYSDGKKKSLNTGIITVQNYGSHVPPKVSHITFAHEVGHNFGSPHDSGTECTPGESKNLGQKENGNYIMYARATSGDKLNNNKFS
Gene ID
Swiss Prot
Synonyms	RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
Calculated MW	84kDa
Observed MW	90kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	RAW264.7
Cellular location	Cell membrane, Endomembrane system, Single-pass type I membrane protein
Customer validation	WB(Mus musculus, Homo sapiens, Rattus norvegicus) IF(Homo sapiens)

Documents

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Research Area

ADAM10 Rabbit pAb images

Western blot - ADAM10 Rabbit pAb (A10438)

Immunofluorescence - ADAM10 Rabbit pAb (A10438)

Immunofluorescence analysis of HeLa cells using ADAM10 antibody (A10438) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for ADAM10 Rabbit pAb (A10438)

Product:

ADAM10 Rabbit pAb

Journal:

Brain Research Bulletin

Application:

IF:

4.077

Species:

Mus musculus

PMID:

32272155

Title:

Morin exerts protective effects on encephalopathy and sepsis-associated cognitive functions in a murine sepsis model

References for ADAM10 Rabbit pAb (A10438)

Product:

ADAM10 Rabbit pAb

Journal:

Chin Med

Application:

IF:

5.455

Species:

Mus musculus

PMID:

33478552

Title:

Suan-Zao-Ren Decoction ameliorates synaptic plasticity through inhibition of the Aβ deposition and JAK2/STAT3 signaling pathway in AD model of APP/PS1 transgenic mice.

References for ADAM10 Rabbit pAb (A10438)

Product:

ADAM10 Rabbit pAb

Journal:

Molecular neurobiology

Application:

IF:

5.59

Species:

Homo sapiens

PMID:

33417226

Title:

Autophagy-Dependent Increased ADAM10 Mature Protein Induced by TFEB Overexpression Is Mediated Through PPARα

References for ADAM10 Rabbit pAb (A10438)

Product:

ADAM10 Rabbit pAb

Journal:

Aging

Application:

IF:

5.95

Species:

Homo sapiens

PMID:

36227154

Title:

Novel small molecular compound 2JY-OBZ4 alleviates AD pathology in cell models via regulating multiple targets - PMC

References for ADAM10 Rabbit pAb (A10438)

Product:

ADAM10 Rabbit pAb

Journal:

Toxicological sciences : an official journal of the Society of Toxicology

Application:

IF:

4.1

Species:

Rattus norvegicus

PMID:

37494465

Title:

Tbx2 Knockdown alleviated sevoflurane-induced cognitive disorder and neuron damages in aged rats via suppressing oxidative stress and ferroptosis

References for ADAM10 Rabbit pAb (A10438)

Product:

ADAM10 Rabbit pAb

Journal:

Biochemical and biophysical research communications

Application:

IF:

3.32

Species:

Homo sapiens

PMID:

37451218

Title:

AMPK boosts ADAM10 shedding activity in human aortic endothelial cells by promoting Rab14-dependent cell surface translocation

References for ADAM10 Rabbit pAb (A10438)

Product:

ADAM10 Rabbit pAb

Journal:

Cancer letters

Application:

IF:

9.75

Species:

Homo sapiens

PMID:

37591356

Title:

Pleckstrin-2 promotes tumour immune escape from NK cells by activating the MT1-MMP-MICA signalling axis in gastric cancer

References for ADAM10 Rabbit pAb (A10438)

Product:

ADAM10 Rabbit pAb

Journal:

Neuron

Application:

IF:

16.2

Species:

Mus musculus

PMID:

37402372

Title:

Hepatic soluble epoxide hydrolase activity regulates cerebral A metabolism and the pathogenesis of Alzheimer's disease in mice

References for ADAM10 Rabbit pAb (A10438)

Product:

ADAM10 Rabbit pAb

Journal:

Brain, behavior, and immunity

Application:

IF:

15.1

Species:

Mus musculus

PMID:

38548184

Title:

Multi-strain probiotics ameliorates Alzheimer's-like cognitive impairment and pathological changes through the AKT/GSK-3 pathway in senescence-accelerated

References for ADAM10 Rabbit pAb (A10438)

Product:

ADAM10 Rabbit pAb

Journal:

Journal of neuroinflammation

Application:

IF:

9.58

Species:

Mus musculus

PMID:

36171620

Title:

Injection of amyloid- to lateral ventricle induces gut microbiota dysbiosis in association with inhibition of cholinergic anti-inflammatory pathways in Alzheimers disease - PMC